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Paul S. Cohen |
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Microbial genetics, the molecular basis of the abilities of enteric bacterial to colonize the mammalian large intestine, the molecular basis of bacterial pathogenesis. The research in this laboratory focuses on bacterial genes involved in the abilities of both pathogenic and non-pathogenic Escherichia coli and Salmonella typhimurium to colonize and/or cause intestinal disease in mice and in the ability of a hydrocarbon degrading strain of Pseudomonas aeruginosa to cause lung disease in mice. Our goals are to make effective bacterial vaccines (e.g. E. coli and S. typhimurium) and to construct bacterial strains which cannot colonize (e.g. P. aeruginosa) and can therefore be released and utilized safely in the environment.
Zhou, X., S.E. , S.E. George, D.W. Frank, M. Utley, I. Gilmour, K.A. Krogfelt, L.D. Claxton, D.C. Laux and P.S. Cohen. 1997. Isolation and characterization of an attenuated strain of Pseudomonas aeruginosa AC869, a 3,5-dichlorobenzoate degrader. Appl. Environ. Microbiol 63:1389-1395 Dobrindt, U., P.S. Cohen, M. Utley, I. Muhldorfer and J. Hacker. 1998. The leuX encoded tRNA5Leu but not the Pathogenicity Islands I and II influence the survival of the uropathogenic Escherichia coli strain 536 in CD-1 mouse bladder mucus in the stationary phase. FEMS Microbiol. Lett. 162:135-141. Utley, M., D.P. Franklin, K.A. Krogfelt, D.C. Laux and P.S. Cohen. 1998. A Salmonella typhimurium strain unable to utilize fatty acids and citrate is avirulent and immunogenic in mice. FEMS Microbiol. Lett. 163: 129-134. Rang, C.U., L.T. Rask, T. Midtvedt, P.L. Conway, L. Chao, K.A. Krogfelt, P.S. Cohen and S. Molin. 1999. Estimation of growth rates of Escherichia coli BJ4 in streptomycin-treated and previously germfree mice by in situ rRNA hybridization. 1999. Clin. Diag. Lab. Immunol. 6:434-436. Krogfelt, K.A., M. Hjulgaard, K. Sorensen, P.S. Cohen and M. Givskov. 2000. RpoS gene function is a disadvantage for Escherichia coli BJ4 during competitive colonization of the mouse large intestine. Infect. Immun. 68:2518-2524. Allen, J.H., M. Utley, H. van den Bosch, P. Nuijten, M. Witvliet, B.A. McCormick, K.A. Krogfelt, T. Rask Licht, D. Brown, M. Mauel, M.P. Leatham, D.C. Laux and P.S. Cohen. 2000. A functional cra gene is required for Salmonella enterica serovar typhimurium virulence in BALB/c mice. Infect. Immun. 68:3772-3775. Krogfelt, K.A., M. Utley, H.C. Krivan, D.C. Laux and P.S. Cohen. 2000. Specific phospholipids enhance the activity of b -lactam antibiotics against Pseudomonas aeruginosa. 2000. J. Antimicrob. Chemother. 46:377-384.
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