The Becton Dickinson FACSCalibur™ is an automated, four-color flow cytometry system capable of analyzing and sorting cells.  Flow cytometry simultaneously measures and then analyzes multiple physical characteristics of cells as they flow in a fluid stream through a beam of light.  The properties measured include relative size, relative granularity or internal complexity, and relative fluorescence intensity.  These characteristics are determined using an optical-to-electronic coupling system that records how the cell scatters incident laser light and emits fluorescence.

Reservation Calendar     ||    Standard Operating Procedure

• Dual laser system providing four color options to analyze and sort a broad range of fluorescent markers
• Three cell sorting modes (single cell, exclusion, recovery)
• Cell capture rate of 300 cells/second
• Operation by a Macintosh Power PC Model #G-4 uniquely configured with system-specific software including BD FACStation™ System Data Management System

Related Resources:

• http://www.bdfacs.com
• http://www.biotech.ufl.edu/FlowCytometry/agronomy/flowdesc.html#definition
• http://www.cytometry.org/
• http://www.cyto.purdue.edu/
• http://www.cyto.purdue.edu/flowcyt/websites/cytsites/sitesusa.htm
• http://pingu.salk.edu/flow/fluo.html
• http://www.probes.com/