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RI-INBRE > Equipment > 3100 OFFGEL Fractionator

3100 OFFGEL Fractionator:

Protein fraction, in-solution recovery

The new Agilent 3100 OFFGEL Fractionator uses a novel isoelectric focusing technique to achieve excellent pl-based fractionation.  The resulting fractions are in solution, making recovery for LC/MS analysis much easier than with old-fashioned gels.

Features of OFFGEL Fractionator

  • pl-based OFFGEL fractionation with 0.1 - 0.6 pH resolution depending on consumable kit used

  • In-solution fraction recovery for easy transfer to LC/MS

  • Two power supplies allow simultaneous fractionation of two sample sets with broad differences in concentration

  • Up to 16 samples fractionated at the same time

  • µg to mg load capacity

  • Possible to run conventional in-gel IEF as well as OFFGEL fractionation

  • Preconfigured methods for OFFGEL and in-gel modes

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