RI-INBRE > Equipment > 3100 OFFGEL Fractionator

3100 OFFGEL Fractionator:

Protein fraction, in-solution recovery

The new Agilent 3100 OFFGEL Fractionator uses a novel isoelectric focusing technique to achieve excellent pl-based fractionation.  The resulting fractions are in solution, making recovery for LC/MS analysis much easier than with old-fashioned gels.

Features of OFFGEL Fractionator

• pl-based OFFGEL fractionation with 0.1 - 0.6 pH resolution depending on consumable kit used

• In-solution fraction recovery for easy transfer to LC/MS

• Two power supplies allow simultaneous fractionation of two sample sets with broad differences in concentration

• Up to 16 samples fractionated at the same time

• µg to mg load capacity

• Possible to run conventional in-gel IEF as well as OFFGEL fractionation

• Preconfigured methods for OFFGEL and in-gel modes