The Jasco J-810 Spectropolarimeter (163-900 nm) is equipped with a Peltier thermoelectric type temperature control system and flow-through HPLC cell.  The instrument is controlled by Jasco’s Spectra Manager™ software.

A circular dichroism (CD) spectropolarimeter measures left- and right-handed circularly polarized light of optically active molecules.  It is a hybrid instrument consisting of a variable wavelength polarimeter and absorption spectrophotometer.

Reservation Calendar     ||    Standard Operating Procedure

• Determination of protein secondary structure
• Determination of optical purity
• Analysis of the tertiary structure of proteins and conformational changes
• Comparison of the secondary and tertiary structures of wild-type and mutant proteins
• Binding studies involving conformational changes
• Nucleic acid structure determination and changes upon binding or melting
• Thermodynamic parameters from temperature dependencies of CD signals

Related Resources:

• http://www.jasco.co.uk/j800.htm
• http://www-structure.llnl.gov/cd/cdtutorial.htm
• http://www.gla.ac.uk/ibls/BMB/np/cdichr/bground.html
• http://www.med.unc.edu/wrkunits/2depts/biochem/MACINFAC/cd.html
• http://www.newark.rutgers.edu/chemistry/grad/chem585/lecture1.html
• http://www.cryst.bbk.ac.uk/BBS/whatis/cd_website.html
• http://www.ap-lab.com/circular_dichroism.htm