|
 Ciphergen’s ProteinChip® System is comprised of a ProteinChip Reader
integrated with ProteinChip Software and a personal computer to analyze
proteins captured on ProteinChip Arrays. The ProteinChip System detects
and accurately calculates the mass of compounds ranging from small
molecules and peptides of less than 1000 Da up to proteins of 500 kDa or
more based on measured time-of-flight.
ProteinChip Arrays
Ciphergen’s
ProteinChip Arrays distinguish this technology from other mass
spectrometry-based analytical systems. ProteinChip Arrays provide a
variety of surface chemistries that allow researchers to optimize
protein capture and analysis. The surface chemistries of the arrays
include a series of classic chromatographic chemistries and specialized
affinity capture surfaces. Classic chromatographic surfaces include
normal phase for generic protein binding; hydrophobic surfaces for
reversed-phase capture; cation- and anion-exchange surfaces; and
immobilized metal affinity capture (IMAC) for metal-binding proteins.
Specific proteins of interest can be covalently immobilized on
pre-activated surface arrays, enabling customized experiments to
investigate antibody-antigen, DNA-protein, receptor-ligand, and other
molecular interactions.
The ProteinChip Reader
The
ProteinChip Reader is a laser desorption/ionization time-of-flight
mass spectrometer that uses state-of-the-art ion optic and laser optic
technology. The laser optics maximize ion extraction efficiency over the
greatest possible sample area, and thus increase analytical sensitivity
and reproducibility. The Reader’s ion optics incorporate a four-stage,
time-lag-focusing ion lens assembly that provides precise, accurate
molecular weight determination with excellent mass sensitivity.
The ProteinChip Software
Ciphergen’s
ProteinChip Software controls all aspects of the ProteinChip Reader
and facilitates data collection and analysis. The software uses a
Microsoft Windows NT interface, and contains numerous features,
including automatic reading of ProteinChip Arrays; multiple spectrum
comparison for differential protein display and biomarker discovery;
several alternative viewing formats for data; and a user-friendly
interface.
The SELDI Process uses a series of ProteinChip Arrays discover,
characterize and develop assays for unique proteins.
Using SELDI technology, the ProteinChip Biology System inbregs new
analytical capabilities such as:
- Quickly resolve mixtures of proteins and protein fragments that
are either too small or have extreme pI’s for gel electrophoresis.
- Develop fast purification methods without columns using a variety
of surface chemistries and buffer conditions.
The ProteinChip Biology System enables you to quickly determine
proteomic changes in response to specific stimuli, and is unique in its
ability to analyze trace (low femtomole) analytes from crude, low-volume
samples.
Related
Resources:
| 
