RI-INBRE > Equipment > Applied
Biosystems Procise® Protein
Sequencing System
Applied Biosystems Procise® Protein
Sequencing System
The Applied Biosystems
Procise® Protein Sequencing System automates the amino acid sequence of
proteins or peptides by the Edman degradation, a chemical process that
removes and analyzes amino acid residues from protein and peptide chains
of various types and length. The instrument delivers volumes of reagents
to a cartridge where the polypeptide is applied to a glass-fiber filter,
which has been previously treated with BioBrene or a PVDF membrane. At
each cycle the PTH-amino acid is transferred to the HPLC for analysis and
quantification. The Protein sequencing system is composed of four
integrated modules: The protein Sequencer, Model 140C Microgradient LC
System, PE 200 UV/VIS Detector and SequencePro™ Software.
