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RI-INBRE > Equipment > Hitachi Semi-Preparative HPLC System > Standard Operating Procedures

Hitachi Semi-Preparative HPLC System
Standard Operating Procedures

The Hitachi analytical HPLC system consists of a L-7100 low pressure gradient pump, 4-channel degasser, L-7200 sequential autosampler, high sensitivity diode array detector (190-800 nm), and a L-7485 fluorescence detector (emission: 200-850 nm; excitation 250-900 nm).  The semi-prep unit is equipped with a L-7150 pump (flow rates 0.1 – 30 mL/min), gradient accessory, 4-channel degasser, several Rheodyne 7725i manual injection valves, L-7455 diode Array detector (flow cell, 3.5 uL), and a Gilson 203B fraction collector. Both systems are controlled by a D-700 HPLC System Manager software package.

Procedure will be available soon.

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