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Standard Operating Procedures
The Hitachi analytical HPLC system consists of a L-7100 low pressure
gradient pump, 4-channel degasser, L-7200 sequential autosampler, high
sensitivity diode array detector (190-800 nm), and a L-7485 fluorescence
detector (emission: 200-850 nm; excitation 250-900 nm). The semi-prep
unit is equipped with a L-7150 pump (flow rates 0.1 – 30 mL/min),
gradient accessory, 4-channel degasser, several Rheodyne 7725i manual
injection valves, L-7455 diode Array detector (flow cell, 3.5 uL), and a
Gilson 203B fraction collector. Both systems are controlled by a D-700
HPLC System Manager software package.
Procedure will be available soon. |