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Molecular and Biochemical Characterization of Lipases Secreted by
Leishmania (2009 - Present)
Investigator:
Alison Shakarian,
Salve
Regina
University
Mentor: Dennis Dwyer, LPD/NIAID
Presentations
2008 - 2009
Shakarian, A.M., McGugan, G., Dwyer, D. 2008.
LdLIP3 encodes a secreted lipase in the human pathogen Leishmania donovani.
American Society of Tropical Medicine and Hygiene 57th Annual Meeting, New
Orleans, LA, December 7-11.
Shakarian, A.M. 2008.
Lipase activity secreted by the human pathogen Leishmania during growth in
vitro is encoded by the LIP3 gene. Rhode Island Research Alliance
Symposium, Providence, RI, June 3.
Shakarian, A.M.,
McGugan, G., Joshi, M., Dwyer, D.M. 2008. Identification and
characterization of the LdLIP3 lipase from the human pathogen Leishmania
donovani. Gordon Research Conference: The Biology of Host-Parasite
Interactions, Newport, RI, June 22-27.
Ganim, C., Byrne, M.,
Shakarian, A.M. 2008. A biochemical characterization of recombinant
lipases secreted by Leishmania. 2nd Biennial IDeA Symposium of Biomedical
Research Excellence, Washington, D.C., August 6-8.
Dodson, B., McEwan,
B., Shakarian, A.M. 2008. Identification and characterization of the gene
encoding a secretory lipase in the human pathogen Leishmania donovani. 2nd
Biennial IDeA Symposium of Biomedical Research Excellence, Washington,
D.C., August 6-8.
Ganim, C., Shakarian,
A.M. 2008. A biochemical characterization of lipases secreted by
Leishmania. New England Association of Parasitologists 8th Annual Meeting,
Boston, MA, October 18.
Byrne, M., Shakarian,
A.M. 2008. Determining the copy number of LdLIP3 transcripts in Leishmania promastigotes using real-time PCR. New England Association of
Parasitologists 8th Annual Meeting, Boston, MA, October 18.
Dodson, B., Shakarian,
A.M. 2008. Identification and characterization of the gene encoding a
secretory lipase in the human pathogen Leishmania donovani. New England
Association of Parasitologists 8th Annual Meeting, Boston, MA, Oct 18.
Shakarian, A.M.,
McGugan, G., Dwyer, D. 2008. A lipase secreted by the human pathogen
Leishmania donovani is encoded by LdLIP3. Keystone Symposium (S4):
Pathogenesis and Control of Emerging Infections and Drug-Resistant
Organisms, Bangkok, Thailand, October 22–27.
Dodson, B., Shakarian,
A.M. 2008. Detection and hybridization of the secretory lipase gene LdLIP3
in Leishmania tarentolae. 3rd Annual BioNES Meeting, Bristol, RI, December
2.
Byrne, M., Shakarian,
A.M. 2008. Determining the copy number of LdLIP3 transcripts using
real-time RT- PCR. 3rd Annual BioNES Meeting, Bristol, RI, December 2.
Stromberg, M.,
Shakarian, A.M. 2008. Southern blot analysis of the LdLIP3 gene loci in
the human pathogen Leishmania donovani. 3rd Annual BioNES Meeting,
Bristol, RI, December 2.
DiLorenzo, J.,
Byrne, M., Shakarian, A.M. 2008. Cloning and sequencing of the beta (β) tubulin
gene encoded in the human pathogen Leishmania donovani. 3rd Annual BioNES
Meeting, Bristol, RI, December 2.
Ganim, C., Shakarian,
A.M. 2008. A biochemical characterization of the enzyme activity encoded
by the secretory lipase gene LdLIP3 in Leishmania donovani. 3rd Annual
BioNES Meeting, Bristol, RI, December 2.
Langley, K., Shakarian,
A.M. 2008. Northern analysis of L. tarenotale total RNA using a secreted
lipase gene specific probe. 3rd Annual BioNES Meeting, Bristol, RI,
December 2.
2007
- 2008 Ganim,
C., Bowers, L., Byrne, M., Shakarian, A.M. 2007. Expression of a
secretory lipase from Leishmania donovani. Biology New England South
Undergraduate Research Symposium, Bristol, RI. Nov 30.
Goulette, R., Shakarian,
A.M. 2007. Isolation and quantification of Leishmania lipase RNA using
real-time PCR. 65th Eastern New England Biology Conference, Boston, MA,
April 22.
Shakarian, A.M.,
McGugan, G., Dwyer, D.M. 2007. The human pathogen Leishmania secretes
lipase activity during growth in vitro. Rhode Island COBRE/INBRE
Symposium, Providence, RI. May 30.
Bowers, L., Shakarian,
A.M. 2007. Secretion of episomally expressed LdLIP3 in Leishmania donovani.
Biology New England South Undergraduate Research Symposium, Roger Williams
University, Bristol, RI. Nov 30.
Bowers, L., Shakarian,
A.M. 2007. Characterization of secretory lipases produced by Leishmania promastigotes. 65th Eastern New England Biology Conference, Boston, MA,
April 22.
Bowers, L., Shakarian,
A.M. 2007. Expression of a secretory lipase gene in Leishmania donovani.
New England Association of Parasitologits 7th Annual Meeting, Boston, MA,
October 20.
Ganim, C., Bowers, L.,
Shakarian, A.M. 2007. Expression of the secretory lipase gene LdLIP3 in
Leishmania tarentolae. New England Association of Parasitologits 7th
Annual Meeting, Boston, MA, October 20.
Greco, J., Shakarian,
A.M. 2007. The expression of a Leishmania secretory lipase gene in E.
coli. 65th Eastern New England Biology Conference, Boston, MA, April 22.
Shakarian, A.M. 2007.
Identification and characterization of lipases secreted by the human
pathogen Leishmania. 2nd Northeast Regional IDeA Meeting, Burlington, VT,
August 15-17.
2005
- 2006
Shakarian, A. 2006. Leishmania: Why study a tropical parasite? Biology
Undergraduate Research Programs Retreat, Providence, RI, December 1, 2006.
Kpea, L., Jamal, A.,
Shakarian, A. M.* A genomics approach to characterize a lipase gene in
Leishmania tarentolae. 1st Biennial National IDeA Symposium of Biomedical
Research Excellence (NISBRE), Washington, DC, July 20-22.
Shakarian, A.M., McGugan
G., Joshi, M., Dwyer, D.M. 2006. Identification, biochemical and molecular
characterization of secreted lipases in the human pathogen Leishmania. The
16th International Congress on Cell Biology and 46th American
Society for Cell Biology Annual Meeting, San Diego, CA, December 9-13.
Garrett, K., Jamal, A.,
Shakarian, A.M. 2006. Identification of the gene encoding a secretory lipase in
the human pathogen Leishmania mexicana. 1st Biennial National IDeA
Symposium of Biomedical Research Excellence (NISBRE), Washington, DC, July
20-22.
Greco, J., Shakarian,
A.M. 2006. The expression of a Leishmania secretory lipase gene in E.
coli. Biology Undergraduate Research Programs Retreat, Providence, RI,
December 1.
Bowers, L., Zuccarelli,
L., Shakarian, A.M. 2006. A biochemical characterization of Leishmania secreted
lipases. 1st Biennial National IDeA Symposium of Biomedical Research
Excellence (NISBRE), Washington, DC, July 20-22.
Bowers, L., Shakarian,
A.M. 2006. Biochemical characterization of Leishmania secreted lipases using a
fluorescent microplate assay. Biology Undergraduate Research Programs
Retreat, Providence, RI, December 1.
Goulette, R., Langley,
K., Shakarian, A.M. 2006. Isolation and quantification of Leishmania lipase RNA
using real-time PCR. Biology Undergraduate Research Programs Retreat,
Providence, RI, December 1.
2004
- 2005
McGugan, G., Shakarian, A., Joshi, M., Dwyer, D. 2005. Identification and
characterization of Leishmania lipases. The 16th Annual International
Molecular Parasitology Meeting, Woods Hole, MA, September 11-15.
McGugan, G., Dwyer, D.,
Shakarian, A. 2005. Characterization of three putative lipase genes in the
human pathogen Leishmania donovani. Northeast Regional IDeA Meeting (INBRE
and COBRE), Mount Desert Island Biological Laboratories, Bar Harbor, ME,
July 17-19.
Medeiros, J., Shakarian,
A. 2005. The identification and characterization of three lipase
genes/proteins from Leishmania using a genomics approach. The 18th Annual
NSF-EPSCoR, National Conference, Rio Grande, Puerto Rico, September 25-29. |
