Mechanisms of CpG-ODN's Protection against UV-induced Cell Death (2009 - Present)

Investigator:  Yinsheng Wan, Providence College

Mentor:  John Marshall, Brown University 

Presentations

2008 - 2009

Cao, C., Lu, S., Kivlin, R., Amaral, A., Song, X., Xu, A., Bi, Z., Chu, W., Kouttab, N., Wan, Y. 2008. UV and H2O2 activate AMPK cell signaling pathway in cultured human skin keratinocytes. Annual Meeting of Society of Investigative Dermatology, Kyoto, Japan, May 11-14.

Cao, C., Lu, S., Kivlin, R., Amaral, A., Song, X., Xu, A., Bi, Z., Chu, W., Kouttab, N., Guan, K., Wan, Y. 2008. mTOR activation protects against UVB-induced cell death in cultured skin keratinocytes and dendritic cells. Annual Meeting of Society of Investigative Dermatology, Kyoto, Japan, May 11-14.

Lu, S., Cao, C., Dragoi, A., Amaral, A., Sowa, A., Song, X., Xu, A., Bi, Z., Chu, W., Kouttab, N., Wan, Y. 2008. SIRT1 activation confers protection against UVB and H2O2-induced cell death in cultured skin keratinocytes. Annual Meeting of Society of Investigative Dermatology, Kyoto, Japan, May 11-14.

Lu, S., Cao, C., Kivlin, R., Amaral, A., Song, X., Xu, A., Bi, Z., Chu, W., Kouttab, N., Wan, Y. 2008. Anti-obesity effects of curcumin are associated with activation of AMPK signaling pathway in 3T3-L1 adipocytes. Annual Meeting of Society of Investigative Dermatology, Kyoto, Japan, May 11-14.

Lu, S., Cao, C., Kivlin, R., Amaral, A., Song, X., Xu, A., Bi, Z., Chu, W., Kouttab, N., Wan, Y. 2008. AMPK activation mediates the de-pigmentation effects of EGF in cultured skin melanocytes. Annual Meeting of Society of Investigative Dermatology, Kyoto, Japan, May 11-14.

Kang, J., Yang, B., Chen, W., Li, Y., Ji, C., Bi, Z., Wan, Y. 2008. Extracellular matrix secreted by senescent fibroblasts induced by UVB promotes cell proliferation in HaCaT cells through PI3K/AKT and ERK signaling pathway. Annual Meeting of Society of Investigative Dermatology, Kyoto, Japan, May 11-14.

Chen, W., Bi, Z., Kang, J., Yang, B., Li Y., Xia, J., Sun, W., Wan, Y. 2008. p53-related apoptosis resistance and tumor suppression activity in UVB-induced premature senescent human skin fibroblasts. Annual Meeting of Society of Investigative Dermatology, Kyoto, Japan, May 11-14.

Cao, C., Dragoi, A., Song, X., Amaral, A., Sowa, A., Lu, S., Chu, W., Kouttab, N., Guan, K., Xu, A., Wan, Y. 2008. mTOR activation protects against UVB-induced cell death in cultured keratinocytes and dendritic cells. Experimental Biology 2008, San Diego, CA, April 5-9.

Cao, C., Dragoi, A., Lu, S., Sowa, A., Kivlin, R., Amaral, A., Hu, G., Chu, W., Di, W., Wan, Y. 2008. Priming with EGFR tyrosine kinase inhibitor and EGF sensitizes ovarian cancer cells to respond to chemotherapeutical drugs. Experimental Biology 2008, San Diego, CA, April 5-9.

Walling, B., Wan, Y. 2008. mTOR activation protects against UVB-induced cell death. 2nd Biennial National IDeA Symposium of Biomedical Research Excellence, Washington D.C., August 6-8.

Kivlin, R., Wan, Y. 2008. UV activates AMPK cell signaling pathway in cultured human skin keratinocytes. 2nd Biennial National IDeA Symposium of Biomedical Research Excellence, Washington D.C., August 6-8.

2007 - 2008

Wan, Y.S. 2007. Modulation of AQP3 and water permeability by retinoic acid in human skin cells. Marshall University School of Medicine, WV, April 5.

Wan, Y.S. 2007. The role of mTOR in protection against UV-induced skin cell damage. INBRE/COBRE Regional Meeting, VT, August 15.

Ji, C., Cao, C., Amaral, A., Lee-Couture, A., Chu, W.M., Kouttab, N., Di, W., Wan, Y.S. 2007. Curcumin down-regulates AQP3 and AQP3-mediated cell migration by inhibiting EGFR activation in ovarian cancer cells. Experimental Biology 2007, Washington, DC, April 15-18.

Cao, C., Ji, C., Amaral, A., Valenti, E., Beveridge, L., O'Brien, C., Bi, Z.G., Hu, G., Kouttab, N., Chu, W.M., Wan, Y.S. 2007. ATP-sensitive potassium channel as a novel target for protection against UV-induced skin immunosuppression. The 68^th Annual SID Meeting, Los Angeles, CA, May 9-12.

Chen, B., Bi, Z.G., Wan, Y.S. 2007. Differential proteomic analysis of human skin fibroblast before and after UVA radiation. The 68th Annual SID Meeting, Los Angeles, CA, May 9-12.

Chen, W.Q., Bi, Z.G., Wan, Y.S. 2007. Repetitive exposures to ultraviolet B radiation causes stress-induced premature senescence of human skin fibroblasts. The 68th Annual SID Meeting, Los Angeles, CA, May 9-12.

Wang, F., Xiang, W.Z., Bi, Z.G., Wan, Y.S. 2007. DNA microarray analysis of local immunity in Condyloma acuminatum lesions. The 68th Annual SID Meeting, Los Angeles, CA, May 9-12.

Wan, Y.S. 2007. Modulation of water channel to rehydrate human skin cells. The 3^rd Hospital of Hangzhou, China, July 6.

Cao, C., Ji, C., Dragoi, A.M., Amaral, A., Lee-Couture, A., Valenti, E., Beveridge, L., O'Brien, C., Kouttab, N., Chu, W.M., Wan, Y.S. 2007. CpG-DNA protects against UV-induced cell damage through induction of interaction between EGFR and DNA-PK and DNA-PK dependent AKT activation in both keratinocytes and dendritic cells. The 68th Annual SID Meeting, Los Angeles, CA, May 9-12.

Ji, C., Cao, C., Beveridge, L., O'Brien, C., Sowa, A., He, L., Bi, Z.G., Hu, G., Kouttab, N., Wan, Y.S. 2007. UVB radiation-induced down-regulation of AQP3 attenuates endocytic activity of cultured dendritic cells. The 68th Annual SID Meeting, Los Angeles, CA, May 9-12. 

Wan, Y.S. 2007. CpG DNA protects UV-induced cell death. The 12th Annual Meeting of Chinese Society of Dermatology, Chongqin, China, June 10-15.

Wan, Y.S. 2007. Modulation of UV-induced down-regulation of aquaprin3 in human skin cells. The 8th International Congress of Cosmetics Dermatology, Beijing, China, October 20.

Cao, C., Wan, S., Amaral, A., Lee-Couture, A., Bi, Z.G., Hu, G., Kouttab, N., Chu, W.M., Wan, Y.S. 2007. All-trans retinoic acid attenuates ultraviolet radiation-induced down-regulation of aquaporin-3 and water permeability in human keratinocytes and fibroblasts. The 68th Annual SID Meeting, Los Angeles, CA, May 9-12.

Cao, C., Ji, C., Amaral, A., Valenti, E., Beveridge, L., O'Brien, C., Sowa, A., Kouttab, N., Chu, W.M., Guan, K.L., Wan, Y.S. 2007. UV- and CpG-ODN-induced S6K activation is mediated by PI3K/AKT/TSC2/mTOR pathway in cultured keratinocytes and dendritic cells. The 68th Annual SID Meeting, Los Angeles, CA, May 9-12.

Ji, C., Cao, C., Beveridge, L., O'Brien, C., Sowa, A., He, L., Bi, Z.G., Hu, G., Kouttab, N., Wan, Y.S. 2007. Activation of AMPK regulates UV-induced phosphorylation and acetylation of p53 in opposite ways in cultured human skin keratinocytes. The 68^th Annual SID Meeting, Los Angeles, CA, May 9-12.

Ji, C., Cao, C., Beveridge, L., O'Brien, C., Sowa, A., He, L., Bi, Z.G., Hu, G., Kouttab, N., Wan, Y.S. 2007. Activation of AMPK regulates UV-induced phosphorylation and acetylation of p53 in opposite ways in cultured human skin keratinocytes. The 68^th Annual SID Meeting, Los Angeles, CA, May 9-12.

Cao, C., Ji, C., Amaral, A., Lee-Couture, A., Chu, W.M., Kouttab, N., Di, W., Wan, Y.S. 2007. EGFR tyrosine kinase activity is not necessary for ligand-induced EGFR down-regulation in ovarian cancer cells. Experimental Biology 2007, Washington, DC, April 15-18.

Cao, C., Ji, C., Amaral, A., Valenti, E., Beveridge, L., O’Brien, C., Bi, Z.G., Hu, G., Kouttab, N., Chu, W.M., Wan, Y.S. 2007. Epidermal growth factor receptor is a critical mediator of ultraviolet radiation-induced signal transduction in dendritic cells. The 68th Annual SID Meeting, Los Angeles, CA, May 9-12.

Ji, C., Cao, C., Beveridge, L., O'Brien, C., Sowa, A., He, L., Bi, Z.G., Hu, G., Kouttab, N., Wan, Y.S. 2007. EGFR is involved in H2O2-induced down-regulation of BCO1 and BCO2 in cultured human skin keratinocytes. The 68th Annual SID Meeting, Los Angeles, CA, May 9-12.

2006 - 2007

Fan, W.X., Dai, Y.Q., Wan, Y.S. 2006. Hypoxia induces expression of hypoxia inducible factor-1 in mouse hair follicles. J Invest Derm, 126(4), 96.

Wang, Q., Bi, Z.G., Zhang, Z.S., Wan, Y.S. 2006. Molecular cloning and expression of E6 and E7 genes of human papillomavirus type 6b. J Invest Derm, 126(4), 127.

Xia, J.P., Bi, Z.G., Wan, Y.S. 2006. Effect of antisense NF-kappaB p65 and EGFR oligodeoxynucleotides on UVB-induced IL6 secretion and c-Jun activation in cultured human skin keratinocytes. J Invest Derm, 126(4), 132.

Xiang, W.Z., Wang, F., Bi, Z.G., Wan, Y.S. 2006. Construction of eukaryotic expression plasmid of HPV11-E7/human IFNα-2b fusion gene and its expression in CHO cell. J Invest Derm, 126(4), 126.

Li, Y.H., Bi, Z.G., Wan, Y.S. (2006) Ultraviolet B radiation induces expression of hypoxia-inducible factor 1α and vascular endothelial growth factor through EGFR/PI3K/AKT Pathway. J Invest Derm, 126(4), 132.

Wan, Y., The role of aquaporins in human skin. The Sino-Japan Joint Conference of Dermatology in Chendu, China, Sept 15-18, 2006.

Wang, X.Y., Bi, Z.G., Wan, Y.S. 2006. Protective effect of gingkgo extracts on UVB-induced premature senescence in human skin fibroblasts. J Invest Derm, 126(4), 133.

Li, YH., Bi, ZG., Wan, Y. 2006. Ultraviolet radiation-induced hypoxia-inducible factor 1 and vascular endothelial growth factor and transferrin receptor expression is dependent on dec1. J Invest Derm, 126(4), 132.

Wan, Y. 2006. The pivotal role of EGFR in ovarian cancer cell migration, Shanghai Second Medical University, China, Sept. 20.

Wang, F., Xiang, W.Z., Bi, Z.G., Li, G.F., Liu, F., Wu, H.W., Zhang, Z.S., Wan, Y.S. 2006. Transfection of dendritic cells with HPV-11 E7 recombinant adenovirus. J Invest Derm, 126(4), 126.

Wu, L., Fan, W.X., Wan, Y.S. 2006. Effect of incubation solution on the viability of hair follicles during hair transplantation. J Invest Derm, 126(4), 96.

Chen, B., Bi, Z.G., Wan, Y. 2006. Differential proteomic analysis of human skin keratinocytes before and after UVB radiation. J Invest Derm, 126(4), 338.

Cao, C., Sun, Y., Bi, Z.G., Hu, G., Chu, W.M., Wan, Y.S. 2006. Pinacidil, an ATP-sensitive potassium channel opener, attenuates Ultraviolet-induced apoptosis in cultured human keratinocytes. J Invest Derm, 126(4), 133.

2005 - 2006

Bi, Z.G., Wang, X.Y., Yan, B.F., Wan, Y.S. 2005. IL-1 receptor antagonist inhibits MMP-1 production of UVA-irradiated human skin fibroblasts cultured with medium from UVB-irradiated keratinocytes. J Invest Dermatol 124, A129.

Chen, B., Bi, Z.G., Yan, B.F., Wan, Y.S. 2005. Protein profiling of human fibroblasts and keratinocytes by two dimensional gel electrophoresis. J Invest Dermatol 124, A59.

Qiu, L.H., Zhou, C.L., Yang, J., Scheffler, E., Derby, S., Bi, Z.G., Yan, B.F., Wanebo, H., Wan, Y.S. 2005. Up-regulation of survivin mediated by transient activation of EGFR/AKT pathway contributes to reduced sensitivity of human melanoma cells to betulinic acid. J Invest Dermatol 124, A139.

Wang, F., Bi, Z.G., Li, G.F., Wu, H.W., Wang, P.A.Q., Liu, F., Wang, X.J., Zhang, Z.S., Yan, B.F., Wan, Y.S. 2005. Generation of Recombinant Adenovirus Vectors of Human Papillomavirus Type 11 E7 Gene and Its Expression in Eukarytoic Cells. J Invest Dermatol 124, A125.

Wang, P., Bi, Z.G., Jing, J., He, J., Yan, B.F., Wan, Y.S. 2005. p16INK4a protein expression and gene inactivation in human skin tumors. J Invest Derm 124, A18.

Wang, X.Y., Bi, Z.G., Yan, B.F., Wan, Y.S. 2005. Protective effect of (-) -epigallocatechin-3-gallate on UVA-induced inhibition of dermal fibroblasts collagen synthesis. J Invest Derm 124, A128.

Xiang, W., Wang, F., Bi, Z.G., Yan, B.F., Wan, Y.S. 2005. Construction and expression of plasmid co-expressing human papillomavirus type 11 E7 and human IFN-2b. J Invest Dermatol 124, A124.

Yang, J., Qiu, L.H., Zhou, C.L., Sheffler, E., Derby, S., Bi, Z.G., Yan, B.F., Wan, Y.S. 2005. DEC1 protects against UV-induced cell death in cultured human keratinocytes. J Invest Dermatol 124, A17.

2004 - 2005

Qiu, L., Di, W., Scheffler, E., Derby, S., Yan, B., Wan, Y. 2005. Ultraviolet irradiation activates MAP kinases via redox-sensitive pathways in cultrued human lens cells. ARVO, April.

2003 - 2004

Wan Y., Yan B. 2003. Intramolecular disulfide bridges are required for folding hydrolase B into a catalytically active conformation. International Society for the Study of Xenobiotics 228.

Xia J.P., Song X.Z., Bi Z.G., Wan Y.S. 2003. Attenuation of UVA-induced human skin keratinocytes damage by (-) epigallocatechin-3-gallate (EGCG). J. Invest. Derm., 1061.

Wang Q., Bi Z.G., Yan B.F., Wan Y.S. 2004. Construction of full length cDNA differential display libraries for genital warts based on long template PCR and full-length cDNA suppression subtractive hybridization (FL-cDNA-SSH). Annual Meeting of Society of Investigative Dermatology, Providence, RI, April 29- May 3. 

Sun W.L., Bi Z.G., Yan B.F., Wan Y.S. 2004. Evaluation of histamine-releasing activity in the sera of patients with chronic urticaria. Annual Meeting of Society of Investigative Dermatology, Providence, RI, April 29 to May 3.

Xia J.P., Song X.Z., Bi Z.G., Wan Y.S. 2003. Attenuation of UV-induced human skin keratinocytes damage by (-) epigallocatechin-3-gallate (EGCG). Annual meeting of the society for Investigative Dermatology, Miami Beach, FL. April 29-May 1.

Wan Y.S. 2003. Cell signaling of UV-induced skin aging and skin cancer. Mount Sinai School of Medicine, NY, Dec. 13.

Yin, J.Q., Gao, J.S., Wan, Y.S., Shao, R.G., Tian, W.G., Wang, J.P. 2003. Effective suppression of proliferation of human cancer cells by combinatorial siRNA molecules. International Society for Investigative Dermatology Annual Meeting, Miami Beach, FL.

Wang F., Bi Z.G., Wang Q., Li G.F., Wang X.J., Zhang Z.S., Yan, B.F., Wan Y.S. 2004. Cloning and expression of E6 and E7 gene of human papilloma virus Type II in E. coli. Annual Meeting of Society of Investigative Dermatology, Providence, RI, April 29-May 3.

Turlington, A., Myne, P., Chiu, C., Blanco, A., Bi, Z.G., Yan, B.F., Wan Y.S. 2004. Insensitivity to oxidative stress and hyper-activation of AKT contribute to anomalous invasiveness of human melanoma cells. Annual Meeting of Society of Investigative Dermatology, Providence, RI, April 29- May 3.

Wan, Y.S., Yan, B.F. 2004. Selective hydrolysis of Rar activator tazarotene by human skin carboxylesterase. Annual Meeting of Society of Investigative Dermatology, Providence, RI, April 29- May 3.

Turlington, A., Chiu, C., Myne, P., Blanco, A., Li, Y.X., Yan, B.F., Wan Y.S. 2003. UV irradiation-induced hypoxia potentiates DEC1/2 and HIF interaction via EGFR and MAP kinase activation. 55th Annual Meeting of Korean Society of Dermatology, Seoul, Korea, October 22-23. 

Wan Y.S. 2003. Molecular mechanism of UV-induced skin cancer. Indiana University, Bloomington, November 15.

Turlington, A., Chiu, C., Myne, P., Bi, Z.G., Li, Y.X., Yan, B.F., Wan, Y.S. 2004. Reciprocal regulation of DEC1 and HIF1a in response to UV irradiation in cultured human keratinocytes. Annual Meeting of Society of Investigative Dermatology, Providence, RI, April 29- May 3.

Chiu, C., Turlington, A., Bruno, M., Myne, P., Preiss, M., Lin, J.Y., Yan, B., Zhang, C.L., Wan, Y.S. 2003. Ligand-independent activation of AKT cell survival pathway under hypoxia contributes to invasiveness of ovarian cancer cells. AACR Annual Meeting, Washington, DC, July 14-19.

Mehta, S., Hokeness, K., Wan, Y.S., Vezeridis, M. 2004. Paclitaxel-induced apoptosis in human keratinocytes is enhanced by IFN-γ and is modulated by activation of caspase-8 and 3 with a concomitant down regulation of the AKT survival pathway. 95th Annual AACR Meeting, Orlando, FL, March 27-31. 

Xia, J.P., Song, X.Z., Bi, Z.G., Yan, B.F., Wan, Y.S. 2004. EGCG inhibits UV-induced NF-kB nuclear translocation and IL-6 secretion in cultured human keratinocytes. Annual Meeting of Society of Investigative Dermatology, Providence, RI, April 29-May 3.

Turlington, A., Myne, P., Blanco, A., Chiu, C., Bi, Z.G., Yan, B.F., Wan, Y.S. 2004. EGFR mediates UV-induced HIF1 expression in cultured human keratinocytes. International Congress of Dermatology. Beijing, China, May 19-13.

Wang, Bi, Z.G., Wang, Q., Li, G.F., Wang, X.J., Zhang, Z.S., Yan, B.F.,  Wan Y.S. 2004. Cloning and expression of E6 and E7 gene of human papilloma virus Type II in E. coli. Annual Meeting of Society of Investigative Dermatology, Providence, RI, April 29- May 3.

Yin, J.Q., Gao, J.S., Wan, Y.S., Shao, R.G., Tian, W.G., Wang, J.P. 2003. Effective suppression of proliferation of human cancer cells by combinatorial siRNA molecules. International Society for Investigative Dermatology Annual Meeting, Miami Beach, FL.

Song, X.Z., Xia, J.P., Bi, Z.G., Yan, B.F., Wan, Y.S. 2004. Aloin attenuates UVA-induced AP1 activation and MMP expression in cultured human skin fibroblasts. Annual Meeting of Society of Investigative Dermatology, Providence, RI, April 29-May 3.

Fan, Zhou N.H., Bi, Z.G., Yan, B.F., Wan, Y.S., Expression of pIRES2-EGFP-hVEGF165 in HaCaT cells and pig hair pappilae cells. Annual Meeting of Society of Investigative Dermatology, Providence, RI, April 29-May 3.

2001 - 2002

Wan, Y. 2002. UV signaling and prevention of UV-induced skin cancer. University of Hong Kong, China, February.

Tao, Y.G., Wan, Y. 2002. EB virus encoded latent membrane protein 1 modulates epidermal growth factor receptor phosphorylation and promoter activity in nasopharyngeal carcinoma cellline, AACR Annual Meeting, San Francisco, CA, April.

Wan, Y. 2002. Investigation of the effects of Chinese herbs on UV-induced skin damage. University of Louisiana Biology Senunar, Lafayette, LA, April.

Heo, S.,  Wan, Y. 2002. Inactivation ofNa+-K+-ATPase and fuctionality of caveolae contribute to cell surface receptor activation that leads to p38 phosphorylation in response to UV irradiation in cultured human keratinocytes. ASMBM Annual Meeting, New Orleans, LA, April.

Garbari, M., Wan, Y. 2002. Anti-inflammatory agent in extracts from Epimedium grandiflorum morro inhibit IL-l-induced expression of IL6 via inhibition of lKK activity and phosphorylation ofp38 in cultured human keratinocytes. ASMBM Annual Meeting, New Orleans, LA, April.