Hemoglobin is a respiratory protein present in the erythrocytes of all vertebrates. Typically, it consists of two identical pairs of α and β globin chains. This project is a continuation of our investigation on snake the Leaf-Nosed Viper (E. macmahonii) hemoglobin from Pakistan. It is being conducted with the intent to characterize and better understand the interspecies relationships among various snakes at the protein level. You will be using various analytical tools in your research such as HPLC/FPLC for protein/peptide purification, MALDI-TOF mass spectrometer to check the purity of isolated protein chains and peptides. The amino acid sequence of α and β chain will be determined by an automated protein sequencer. The deduced amino acid sequence of the Viper globin chains will be aligned to other reported snake hemoglobin sequences.