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Real-time or Quantitative PCR

For internal and RI-EPSCoR users, there is no fee to use either the Stratagene Mx3005P or the Roche LightCycler® 480 real-time qPCR Systems.
User supplied consumables:
Primers
DNA or RNA template
qPCR reagent kit (for RT-qPCR, SYBR® Green protocols or any custom designed fluorescent probe such as TaqMan® or other dual-labeled probe, Scorpions, and/or Molecular Beacons)
Stratagene optical plates/tubes and caps
Roche clear or white multiwell plates and seals
The GSC has a limited supply of Stratagene optical plates, tubes and caps and Roche multiwell plates and seals that can be used for preliminary tests.
New users of qPCR are encouraged to follow the Standard Operating Procedures for this method that are listed under the Procedures menu. These methods work well and have been used repeatedly with excellent results.
To ensure consistent experimental results between the two qPCR systems, we recommend that users follow the standardized MIQE nomenclature guidelines for publication of quantitative real-time PCR experiments: Bustin, S.A. et al. 2009. "The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments." Clinical Chemistry 55(4) 611-622.
reserve time to the use one of the qPCR instruments, please use the GSC Calendar. If you have any questions or problems, call the GSC manager at 874-5919.
Stratagene's "Introduction to Quantitative PCR - Methods and Application Guide" is an excellent introduction to QPCR. You can find this guide at the following link: http://www.stratagene.com/tradeshows/fasttrack.aspx
Please contact the RIGSC for information on the current discounted pricing for reagent kits and consumables from Stratagene and Roche.